Used in biomedical research to quantify autophagy levels in mouse cells and tissues. The kit enables detection of LC3-II, a protein that associates with autophagosomal membranes, making it a key biomarker for autophagosome formation. Researchers use it to study autophagy in various disease models such as neurodegenerative disorders, cancer, and infectious diseases. It is commonly applied in drug development and toxicity studies where modulation of autophagy is a target or concern. The ELISA format allows for high-throughput screening and precise measurement of LC3-II levels in multiple samples simultaneously.