Used in biomedical research to quantify levels of apoptosis signal-regulating kinase 1 (ASK-1) in cell lysates, tissue extracts, or biological fluids. Helps investigate cellular stress responses, inflammation, and programmed cell death pathways. Commonly applied in studies related to neurodegenerative diseases, cardiovascular conditions, and cancer, where ASK-1 signaling plays a key role. Enables researchers to assess the effectiveness of therapeutic compounds targeting ASK-1 activity in experimental models.