Used in research settings to quantify Klotho protein levels in biological samples such as serum, plasma, and cell culture supernatants. Enables studies on aging, kidney function, and age-related diseases including osteoporosis, cardiovascular disorders, and neurodegenerative conditions. Supports investigation into the role of Klotho in metabolic regulation and cellular protection. Commonly applied in preclinical studies and biomarker validation in academic and pharmaceutical research.