Used in biomedical research to quantify levels of LC3 protein, a key marker for autophagy. Enables researchers to monitor autophagosome formation and assess autophagy activity in cell and tissue samples. Commonly applied in studies related to cancer, neurodegenerative diseases, and cellular stress responses. Provides a reliable, sensitive, and high-throughput method for evaluating the effects of drugs, genetic modifications, or environmental factors on autophagy pathways. Widely used in both basic research and drug development settings.