Used in biomedical research to quantify myosin light chain kinase (MLCK) levels in human biological samples such as serum, plasma, or tissue homogenates. This ELISA kit enables researchers to study MLCK's role in cellular processes like muscle contraction, cytoskeletal regulation, and endothelial barrier function. It is particularly valuable in inflammation, asthma, and cardiovascular disease studies where MLCK activity influences vascular permeability and smooth muscle dynamics. The assay provides high sensitivity and specificity, allowing accurate measurement of MLCK concentration in experimental and clinical samples to support mechanistic insights and biomarker development.