Used in biomedical research to detect and quantify nuclear transcription factor kappa B (NF-κB) in rat samples. This kit is essential for studying inflammatory responses, immune system regulation, and cellular stress pathways. Researchers use it in preclinical studies to evaluate the effects of drugs, toxins, or therapeutic agents on NF-κB activation in tissues or cultured cells. It supports investigations in diseases such as arthritis, sepsis, cancer, and neurodegenerative disorders where NF-κB signaling plays a key role. The assay enables high-throughput analysis in microplate format, providing sensitive and specific measurement of NF-κB levels without requiring radioactivity or complex equipment.