Used in biomedical research to quantify protein disulfide isomerase (PDI) levels in cell lysates, tissue extracts, and biological fluids. Enables investigation of endoplasmic reticulum stress, unfolded protein response, and oxidative protein folding in diseases such as cancer, neurodegenerative disorders, and diabetes. Supports drug development by assessing PDI expression changes in response to therapeutic compounds. Facilitates high-throughput screening in academic and pharmaceutical laboratories due to its sensitivity and reproducibility.