Used in biomedical research to quantify substance P receptor (neurokinin-1 receptor) activity in cell cultures, tissue samples, or biological fluids. Helps investigate neuroinflammatory processes, pain signaling pathways, and neurological disorders such as migraine, depression, and Parkinson’s disease. Commonly applied in preclinical studies to evaluate the efficacy of receptor-targeting drugs, including antagonists for pain management or psychiatric conditions. Also utilized in studying gut-brain axis interactions, neurogenic inflammation, and stress responses. Provides a reliable, high-throughput method for measuring receptor expression and ligand binding in experimental models.