Used in biomedical research to quantify levels of peroxisome proliferator-activated receptor alpha (PPAR-α) in rat tissue samples or cell lysates. This assay helps investigate the role of PPAR-α in metabolic pathways, particularly in lipid metabolism, inflammation, and energy homeostasis. Commonly applied in studies related to fatty liver disease, diabetes, and cardiovascular disorders to assess how experimental treatments modulate PPAR-α expression. Enables researchers to evaluate drug efficacy and toxicity in preclinical models by providing a sensitive and specific measurement of PPAR-α protein concentration.