Used in biomedical research to quantify Smad7 protein levels in cell lysates or tissue samples, aiding studies on TGF-β signaling pathways. Helps investigate inflammatory diseases, fibrosis, and cancer progression by measuring Smad7 expression changes in response to stimuli. Supports drug development by evaluating therapeutic effects on intracellular signaling regulation. Commonly applied in immunology and molecular biology labs for high-throughput screening and mechanistic studies.