Used in biomedical research to quantify levels of Sonic Hedgehog (SHH) protein in cell lysates, tissue extracts, and biological fluids such as serum or cerebrospinal fluid. Enables researchers to study SHH signaling pathway activity, which plays a critical role in embryonic development, tissue patterning, and stem cell regulation. Widely applied in cancer research, particularly in investigating tumor growth and progression in cancers like medulloblastoma and basal cell carcinoma where SHH signaling is dysregulated. Also used in neuroscience to assess neural development and in regenerative medicine to monitor differentiation of stem cells. The kit supports high-throughput screening of compounds that modulate the Hedgehog pathway, aiding drug discovery and validation.