Used in biomedical research to quantify phosphorylated SMAD2 protein levels in cell or tissue samples. Helps study TGF-β signaling pathway activity, which plays a key role in cell growth, differentiation, and apoptosis. Widely applied in cancer research, fibrosis studies, and immune regulation to assess pathway activation under experimental or pathological conditions. Enables high-throughput screening of drug candidates targeting TGF-β-related mechanisms. Provides a reliable, sensitive method for detecting changes in SMAD2 phosphorylation, supporting both basic science and therapeutic development.