Used in biomedical research to quantify levels of sterol regulatory element binding protein 1 (SREBP-1) in rat tissue samples, particularly in studies related to lipid metabolism, insulin resistance, and fatty liver disease. Enables researchers to assess activation and expression of SREBP-1 in response to dietary, pharmacological, or genetic interventions. Commonly applied in metabolic syndrome and diabetes research to understand molecular mechanisms regulating fatty acid and cholesterol synthesis. Provides a reliable, sensitive method for measuring nuclear and precursor forms of SREBP-1 in cell lysates or tissue homogenates.