Used in biomedical research to measure levels of SIRT3 protein in rat tissue samples, particularly in studies related to mitochondrial function, cellular metabolism, and aging. Enables researchers to investigate the role of SIRT3 in oxidative stress responses, neurodegenerative diseases, cardiovascular conditions, and metabolic disorders such as diabetes. Commonly applied in preclinical studies using rat models to evaluate potential therapeutic interventions targeting sirtuin pathways. Provides a reliable, sensitive, and quantitative method for detecting SIRT3 in cell lysates and tissue homogenates, supporting mechanistic studies in molecular biology and pharmacology.