Used in biomedical research to quantify levels of Human Topoisomerase II alpha (TOP2a) protein in cell lysates, tissue extracts, or biological fluids. This ELISA kit enables researchers to study TOP2a expression in cancer cells, particularly in breast, ovarian, and leukemia cancers, where TOP2a is often overexpressed and linked to tumor progression and response to chemotherapy. It supports investigation into the efficacy and mechanisms of topoisomerase-targeting drugs such as doxorubicin and etoposide. The assay is also applied in molecular oncology and pharmacology studies to monitor changes in TOP2a during cell cycle phases, DNA repair processes, and apoptosis. Suitable for high-throughput screening and biomarker validation in clinical and preclinical research settings.