Used in research to quantify uncoupled protein 1 (UCP1) levels in rat tissue samples, particularly in studies related to brown adipose tissue (BAT) function and thermogenesis. Helps investigate metabolic disorders, obesity, and energy expenditure mechanisms. Commonly applied in preclinical studies involving animal models to assess the impact of drugs, diets, or genetic modifications on mitochondrial uncoupling and heat production. Provides a reliable, sensitive method for detecting UCP1 protein concentration in cell lysates or tissue homogenates, supporting endocrinology and metabolism research.