Used in biomedical research to quantify levels of ZO-1 protein in cell or tissue samples, aiding in the study of epithelial and endothelial barrier integrity. Commonly applied in research on inflammatory diseases, cancer, and organ-specific conditions such as intestinal permeability, blood-brain barrier dysfunction, and kidney or lung injury. Enables researchers to assess tight junction disruption or preservation under experimental conditions, supporting drug development and mechanistic studies in cell signaling and barrier function.