RIPA Lysis Buffer (Medium) is widely used in molecular biology and biochemistry for the extraction of proteins from cells and tissues. Its primary application is in the preparation of cell lysates for subsequent analysis, such as Western blotting, immunoprecipitation, and protein quantification assays. The buffer effectively disrupts cell membranes and solubilizes proteins while maintaining their native state, making it ideal for studying protein-protein interactions, post-translational modifications, and signaling pathways. Additionally, it is commonly employed in research involving cancer biology, immunology, and neuroscience to isolate proteins from complex biological samples. Its versatility and efficiency make it a staple in laboratories focused on protein analysis and characterization.