RIPA Lysis Buffer (Strong) is widely used in molecular biology and biochemistry for the efficient extraction of proteins from cells and tissues. Its strong formulation is particularly effective in breaking down cell membranes and nuclear membranes, making it suitable for isolating both cytoplasmic and nuclear proteins. This buffer is commonly employed in techniques such as Western blotting, immunoprecipitation, and protein assays, where high-quality protein samples are essential. Additionally, it helps in solubilizing membrane-bound proteins, which can be challenging to extract with milder buffers. The inclusion of detergents and inhibitors in the buffer ensures the preservation of protein integrity by preventing degradation and maintaining biological activity during the extraction process.