RIPA Lysis Buffer is widely used in molecular biology and biochemistry for the extraction of proteins from cells and tissues. Its primary application is in preparing protein samples for analysis techniques such as Western blotting, immunoprecipitation, and ELISA. The buffer effectively lyses cells, breaking down cell membranes and releasing intracellular proteins while maintaining their integrity and functionality. It is particularly useful for extracting both soluble and membrane-bound proteins, making it a versatile tool in protein research. Additionally, RIPA Lysis Buffer is often employed in studying protein-protein interactions, post-translational modifications, and signal transduction pathways. Its ability to inhibit proteases and phosphatases ensures that the extracted proteins remain stable and unaltered during the experimental process.