Phospho-Protein Extraction Buffer IV is widely used in biochemical research for the efficient extraction of phosphorylated proteins from cell and tissue samples. Its primary application is in the study of cell signaling pathways, where it helps isolate phospho-proteins while maintaining their phosphorylation state. This buffer is particularly useful in Western blotting, immunoprecipitation, and mass spectrometry experiments, enabling researchers to analyze protein phosphorylation events critical for understanding cellular processes like growth, differentiation, and apoptosis. Its optimized formulation ensures minimal protein degradation and high yield, making it a reliable tool for phospho-proteomics studies.