The Two-Color Prestained Protein Ladder (15-130kDa) is widely used in molecular biology and biochemistry laboratories for protein analysis. Its primary application is in SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis), where it serves as a reference marker to estimate the molecular weights of unknown proteins. The prestained bands allow researchers to visually monitor the progress of electrophoresis in real-time, ensuring accurate separation and analysis. This ladder is particularly useful for Western blotting, as the prestained proteins facilitate the transfer efficiency assessment from the gel to the membrane. The two-color system enhances band visibility, making it easier to distinguish between different molecular weight markers during both gel running and blotting processes. Additionally, it is employed in quality control procedures to validate the performance of electrophoresis systems and ensure consistency in protein separation. Its broad range (15-130kDa) makes it suitable for analyzing a wide variety of proteins, from small peptides to larger complexes, making it a versatile tool in protein research and diagnostics.