Prestained Protein Ladder (10-130kDa) is widely used in molecular biology and biochemistry laboratories for protein analysis. Its primary application is in SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis), where it serves as a reference marker to estimate the molecular weights of unknown proteins. The prestained bands allow researchers to visually track the progress of electrophoresis in real-time, ensuring accurate separation and analysis. This ladder is particularly useful for verifying the efficiency of protein transfer during Western blotting, as the prestained proteins can be directly observed on the membrane. It simplifies the identification of target proteins by providing a clear size comparison, enhancing the reliability of experimental results. Additionally, it is a valuable tool for troubleshooting gel runs and optimizing experimental conditions.