N-Hydroxysuccinimide is widely used in bioconjugation and protein chemistry. It is a key reagent in the formation of stable amide bonds, particularly in the activation of carboxyl groups for coupling with amines. This makes it essential in the preparation of peptide and protein conjugates, such as antibody-drug conjugates used in targeted cancer therapies. It is also employed in the modification of biomolecules for immobilization on surfaces, which is crucial in biosensor development and diagnostic assays. Additionally, it plays a role in crosslinking reactions for studying protein interactions and in the synthesis of polymers for drug delivery systems. Its versatility and efficiency in coupling reactions make it a valuable tool in both research and industrial applications.