SEC inhibitor KL-1 is primarily used in biochemical research to study the mechanisms of protein translocation and secretion in cells. It is particularly valuable in investigating the Sec61 translocon complex, which plays a critical role in the transport of proteins across the endoplasmic reticulum membrane. Researchers utilize this inhibitor to analyze the effects of blocking protein secretion pathways, aiding in the understanding of diseases related to protein misfolding or improper trafficking, such as cystic fibrosis or certain types of cancer. Additionally, it serves as a tool in drug discovery to identify potential therapeutic targets by disrupting protein secretion processes in experimental models.