Amido Black 10B is widely used in biological and biochemical research as a staining agent for proteins. It is particularly effective in visualizing proteins on nitrocellulose or polyvinylidene fluoride (PVDF) membranes after electrophoresis. The dye binds to proteins, producing a dark blue or black color, which helps in identifying and quantifying protein bands. This makes it a valuable tool in Western blotting techniques, where protein detection and analysis are critical. Additionally, Amido Black 10B is sometimes used in histology for staining tissue sections, aiding in the microscopic examination of cellular structures. Its strong affinity for proteins also makes it useful in forensic science for detecting protein residues in fingerprint analysis.