Amido Black 10B is a versatile azo dye used both in industrial and scientific applications. In the textile industry, it is employed for dyeing natural fibers such as cotton and linen, providing an intense and durable black color that enhances aesthetic appeal and color fastness.

In biological and biochemical research, it serves as a staining agent for proteins, particularly effective for visualizing proteins on nitrocellulose or polyvinylidene fluoride (PVDF) membranes following electrophoresis. The dye binds to proteins, yielding a dark blue or black coloration that facilitates the identification and quantification of protein bands. This renders it essential in Western blotting techniques for protein detection and analysis. Furthermore, Amido Black 10B is utilized in histology to stain tissue sections, supporting microscopic examination of cellular structures. Its affinity for proteins also finds application in forensic science for detecting protein residues in fingerprint analysis.