Pentedrone hydrochloride is primarily used in research settings to study its effects on the central nervous system, particularly its stimulant properties. It has been investigated for its potential to influence dopamine and norepinephrine levels, making it relevant for understanding neurotransmitter dynamics and related disorders. In forensic science, it is analyzed to identify and differentiate it from other similar compounds in legal and regulatory contexts. Additionally, it has been explored in behavioral studies to assess its impact on activity patterns and addiction mechanisms in animal models. Its use is strictly controlled due to its potential for abuse and lack of approved medical applications.