Used primarily in biochemical research, 2,4-Pteridinediol serves as a key intermediate in the synthesis of various pteridine derivatives. These derivatives are crucial for studying enzyme mechanisms, particularly those involving folate metabolism. The compound is also employed in the development of potential therapeutic agents targeting diseases related to folate pathways, such as certain cancers and autoimmune disorders. Additionally, it finds application in the preparation of fluorescent dyes and markers used in cellular imaging and diagnostic assays. Its role in these areas underscores its importance in advancing both medical research and diagnostic technologies.