Used in biochemical research, this compound is often applied in studies related to enzyme activity, particularly as a cofactor in enzymatic reactions involving amino acid metabolism. It plays a role in investigating the function of enzymes like phenylalanine hydroxylase, which is crucial for understanding metabolic disorders such as phenylketonuria. Additionally, it is utilized in the synthesis of tetrahydrobiopterin analogs, aiding in the development of therapeutic agents for conditions linked to neurotransmitter synthesis and nitric oxide production. Its application extends to the study of oxidative stress and cellular signaling pathways, providing insights into disease mechanisms and potential treatment strategies.