2,4-Diaminopyrimidin-5-ol dihydrochloride is widely utilized in pharmaceutical chemistry, particularly in the synthesis and development of anti-cancer drugs. Its structure plays a crucial role as a key intermediate in designing molecules that inhibit thymidylate synthase (TS), an enzyme essential for DNA synthesis in cancer cells. Additionally, it is employed in studies for developing antimicrobial agents, especially against bacteria and fungi, due to its effective inhibition of microbial growth. This compound supports biochemical research and the creation of therapeutic agents targeting complex diseases such as cancer and infections.