Used in research to study axon guidance and neural development due to its role in the Slit-Robo signaling pathway. Facilitates protein-protein interaction studies, especially in understanding cell migration and nervous system patterning. The His Tag allows for easy purification and detection in recombinant expression systems. Commonly applied in ELISA, Western blotting, and immunoprecipitation assays to investigate receptor-ligand interactions. Also used in structural studies to determine binding mechanisms with Slit proteins.