Used in immunological research to study Toll-like receptor 7 (TLR7) signaling pathways involved in innate immunity. Serves as a target for drug development in antiviral therapies and autoimmune diseases due to its role in recognizing single-stranded RNA. The His tag enables easy purification and detection in recombinant protein applications. Employed in ELISA, Western blotting, and protein-protein interaction studies to investigate immune responses and screen potential therapeutic compounds.