Used in research and development of vaccines and diagnostic tools targeting the Monkeypox virus. The A35R protein plays a role in viral entry and immune modulation, making it a key antigen for studying immune responses. The mFc tag allows for easy detection and purification in laboratory settings, facilitating studies on antibody binding, protein interactions, and vaccine efficacy. Commonly used in ELISA, Western blot, and neutralization assays to evaluate monoclonal antibodies or sera from vaccinated individuals.