Mouse SFRP3, Tag Free is primarily used in biochemical and cellular research to study Wnt signaling pathways. It serves as a critical tool for investigating cell differentiation, embryonic development, and tissue homeostasis. Due to its role as a secreted frizzled-related protein, it modulates Wnt activity by binding to Wnt ligands or frizzled receptors, making it valuable in studies related to cancer, stem cell regulation, and regenerative medicine. Researchers use the tag-free version in protein-protein interaction assays, receptor binding studies, and structural analysis where the absence of affinity tags ensures native conformation and avoids potential interference in functional assays. It is also employed in in vitro and in vivo models to examine joint development and cartilage formation, particularly in the context of skeletal disorders.