Used in research to study Wnt signaling pathways, particularly in developmental biology and cancer research. Helps in understanding receptor-ligand interactions involving Frizzled family proteins. Commonly applied in protein-protein interaction assays, ELISA, and antibody development due to the His tag facilitating purification and detection. Also utilized in structural studies and binding assays to explore therapeutic targets related to eye development and diseases like familial exudative vitreoretinopathy (FEVR).