This chemical is primarily used in immunological research, particularly in studies involving γδ T cell activation. It serves as a critical ligand for the BTN3A1 receptor, playing a key role in phosphoantigen recognition, which is essential for triggering immune responses. Its application extends to cancer immunotherapy development, where modulation of γδ T cells can enhance anti-tumor activity. It is also utilized in cellular assays to investigate immune cell signaling pathways and in the development of biologics targeting the CD277 pathway. Due to its expression in HEK293 cells, it is commonly employed in recombinant protein production and binding studies for drug screening.