This recombinant protein is primarily used in immunological research, particularly in studies involving gamma delta (γδ) T cell activation and regulation. It serves as a critical target for understanding immune responses, as it plays a role in phosphoantigen recognition by Vγ9Vδ2 T cells, the major subset of human γδ T cells. Due to its expression in HEK293 cells, it is often employed in functional assays, protein interaction studies, and antibody validation. It is also utilized in the development and testing of therapeutic agents such as bispecific antibodies, CAR-T cells, and other immunotherapies aimed at enhancing anti-tumor immune responses. Its application extends to drug screening platforms where modulation of the BTN3A1 pathway is required to assess immune activation.