This recombinant protein is primarily used in immunological research to study the role of the CD96 receptor in immune cell signaling and tumor immunity. It serves as a critical tool for investigating the interactions between immune cells and cancer cells, particularly in the context of the tumor microenvironment. Researchers use it to explore the balance between immune activation and suppression mediated by the CD96 pathway, often in comparison with related receptors like TIGIT and CD226. It is also employed in in vitro binding assays and functional studies to evaluate potential immunotherapeutic agents targeting the CD96 pathway. Its expression in HEK293 cells ensures proper post-translational modifications, making it suitable for structural and functional analyses.