huSiglec-9-HEK293 is a recombinant human Siglec-9 protein produced in HEK293 cells. It is primarily used in immunological research to study immune cell regulation, particularly the role of Siglec-9 in modulating innate immune responses. This protein is applied in binding assays to identify ligands that interact with Siglec-9, especially sialic acid-containing glycans found on pathogens or tumor cells. It is also used to investigate inhibitory signaling pathways in myeloid cells such as neutrophils and macrophages, which can influence inflammation and immune tolerance. In cancer research, huSiglec-9-HEK293 helps explore tumor immune evasion mechanisms, as many cancers upregulate sialylated structures to engage Siglec-9 and suppress immune attack. Additionally, it serves as a tool for screening therapeutic agents like blocking antibodies or glycan mimetics aimed at disrupting Siglec-9-mediated immunosuppression.