This recombinant protein is primarily used in immunological research to study the signaling pathways involved in inflammation and immune response regulation. It serves as a critical tool for investigating the ST2/IL-33 pathway, which plays a key role in Th2-mediated immune responses, allergic inflammation, and cardiovascular diseases. Due to its functional interaction with NF-κB signaling, it is employed in cell-based assays to examine inflammatory gene expression and pathway activation. Commonly expressed in HEK293 cells, this protein is utilized in vitro to assess receptor-ligand interactions, screen potential therapeutic inhibitors, and develop immunoassays for biomarker detection, especially in heart failure and asthma research.