Prostaglandin F2α isopropyl ester is primarily used in veterinary medicine for its luteolytic properties. It is commonly administered to induce estrus in livestock, such as cows and horses, by regressing the corpus luteum and synchronizing the reproductive cycle. This application is particularly valuable in breeding programs to optimize timing and improve reproductive efficiency. Additionally, it is utilized in the treatment of pyometra, a uterine infection in animals, by promoting the expulsion of uterine contents. In human medicine, it has been explored for its potential in managing glaucoma due to its ability to reduce intraocular pressure, though its use in this context is less common.