Used for quantitative determination of estradiol (E2) levels in human serum, plasma, or other biological fluids. Widely applied in clinical research and diagnostic laboratories to study hormonal imbalances, reproductive health, fertility disorders, and endocrine-related diseases. Supports research in menopause, ovarian function, and hormone replacement therapy. The assay enables high-throughput analysis with high sensitivity and specificity, making it suitable for large-scale epidemiological studies and biomedical research involving estrogen metabolism.