Used in research settings to measure free testosterone levels in mouse serum or plasma samples. This ELISA kit enables scientists to quantify the biologically active fraction of testosterone that is not bound to proteins, providing insight into endocrine function and hormonal status in preclinical studies. It is commonly applied in studies related to reproductive health, metabolic disorders, aging, and hormone-dependent diseases in mouse models. The assay offers high sensitivity and specificity, allowing for accurate detection of small changes in free testosterone concentrations, which is critical for evaluating the effects of drugs, genetic modifications, or environmental factors on the male or female mouse endocrine system.