Used in reproductive biology research to quantify gonadotropin-releasing hormone levels in rat samples. Enables study of hormonal regulation in the hypothalamic-pituitary-gonadal axis. Commonly applied in experiments assessing fertility, puberty, stress effects on reproduction, and endocrine disruption. Provides a sensitive and specific measurement of GnRH in tissue homogenates, serum, or cell culture supernatants. Supports investigation of drug effects, genetic modifications, or environmental factors on reproductive hormone secretion in rodent models.