Used in research settings to measure testosterone levels in mouse biological samples such as serum, plasma, or tissue homogenates. This assay enables endocrinologists and biomedical researchers to study hormonal changes related to stress, reproduction, metabolism, and behavior in mouse models. Commonly applied in studies investigating the hypothalamic-pituitary-gonadal axis, the effects of drugs or environmental factors on hormone levels, and developmental or gender-related differences in hormone expression. The kit provides a sensitive and specific method for quantifying testosterone, supporting data accuracy in preclinical research.