Endonuclease SphI is widely used in molecular biology for DNA manipulation. It is a restriction enzyme that specifically recognizes and cleaves DNA at a particular sequence, making it valuable for genetic engineering and cloning experiments. Researchers use it to cut DNA into precise fragments, which are then analyzed or inserted into vectors for further study. It is also employed in restriction mapping to determine the structure of DNA molecules. Additionally, SphI is utilized in the construction of recombinant DNA, aiding in the study of gene function and regulation. Its specificity and efficiency make it a crucial tool in biotechnology and genetic research.