Guvacine ethyl ester is primarily utilized in neurological research due to its role as a selective inhibitor of GABA uptake. It is often employed in studies focused on understanding the mechanisms of GABAergic neurotransmission, which plays a critical role in regulating brain excitability. By inhibiting GABA reuptake, it enhances GABAergic activity, making it a valuable tool in exploring conditions related to GABA dysfunction, such as anxiety, epilepsy, and sleep disorders. Additionally, it is used in pharmacological experiments to investigate the therapeutic potential of GABA modulation in neurological and psychiatric disorders. Its application extends to in vitro and in vivo models, helping researchers dissect the complexities of GABAergic signaling pathways.