5(6)-TAMRA is widely used as a fluorescent dye in various biological and chemical applications. Its bright orange-red fluorescence makes it ideal for labeling peptides, proteins, and nucleic acids in fluorescence microscopy, flow cytometry, and fluorescence resonance energy transfer (FRET) studies. The dye is particularly useful in live-cell imaging due to its photostability and compatibility with common fluorescence filters. It is also employed in molecular biology for tagging oligonucleotides in fluorescence in situ hybridization (FISH) and as a tracer in biochemical assays. Additionally, its carboxylic acid group allows for easy conjugation to biomolecules, making it a versatile tool in bioconjugation and diagnostic applications.