This chemical is widely used as a fluorescent dye in biological and biochemical research. It is particularly valuable for labeling proteins, peptides, and other biomolecules due to its high fluorescence intensity and stability. The succinimidyl ester group allows for efficient covalent bonding with primary amines, making it ideal for conjugation applications. It is commonly employed in fluorescence microscopy, flow cytometry, and immunofluorescence assays to visualize and track cellular components. Additionally, it is used in molecular biology techniques such as DNA sequencing and fluorescence in situ hybridization (FISH) to enhance detection sensitivity. Its water solubility and compatibility with various experimental conditions make it a versatile tool in life sciences.