Used extensively as a fluorescent dye in biological research, it helps in labeling and tracking molecules within cells. Its high fluorescence intensity makes it ideal for applications in flow cytometry and fluorescence microscopy. The dye is also employed in immunofluorescence studies to visualize specific proteins or structures in tissues and cells. Additionally, it serves as a useful tool in molecular biology for detecting nucleic acids in gel electrophoresis. Its stability and bright emission make it a preferred choice for live-cell imaging and diagnostic assays.